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A unique PCR troubleshooting guide that is an essential companion for anyone who uses the polymerase chain reaction technique.

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Troubleshooting Guides; The Polymerase Chain Reaction (PCR) Taq DNA Polymerase is an enzyme widely used in PCR (2).

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The following points should be considered when designing PCR primers and are common to all types of PCR: T m calculation: 2°C x (A+T) + 4°C x (G+C)

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